Irrespective of the type of syringe and needle.ĭo not puncture the vial stopper more than 20 times.Primary series doses from the dark blue cap vial must contain 0.25 mL of vaccine.Primary series doses from the red cap vial must contain 0.5 mL of vaccine.Booster doses from the red cap vial must contain 0.25 mL of vaccine.Booster doses from the dark blue cap vial must contain 0.5 mL of vaccine.